Reporter

Part:BBa_K1114503:Design

Designed by: Jake Awtry   Group: iGEM13_BostonU   (2013-09-12)


MoClo Level 1 RFP reporter with AE fusion sites


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 710
    Illegal AgeI site found at 822
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

pJ04B12Rm_AE

This is a MoClo Level 1 reporting device containing the Level 0 parts J23104_AB, BCD12_BC, E1010m_CD, and B0015_DE within the DVL1_AE backbone. The flanking fusion sites are A on the 5' side and E on the 3' side of the insert. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. In the given sequence the internal fusion sites are incorrectly duplicated. See Level 0 pages for further information.

Source

Construct of MoClo Level 0 parts derived from iGEM Distribution Kits


References